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mouse  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc mouse
    Mouse, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 696 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phospho+histone+h3/Phospho-Histone+H3+(Ser10)+Mouse+mAb/pmc13050020-8-3-5
    Average 96 stars, based on 696 article reviews
    mouse - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    other:

    Article Title: Therapeutic synergies that overcome carboplatin resistance in triple-negative breast cancer
    Article Snippet: Phospho-Histone H3 , Rabbit IgG , H, M, R, Mk, Dm , 17 , 1:200 , Cell Signaling Technology , #9701.

    Immunostaining:

    Article Title: Phosphorylations of serines 21/9 in glycogen synthase kinase 3α/β are dispensable for V600EBRAF-driven premalignant tumour development in the mouse intestine.
    Article Snippet: .. Primary antibodies used for immunostaining were: BrdU (Cell signalling, 5292, 1:500), phospho-histone H3 (Cell signalling, 9701, 1:500) and β-catenin (Sigma, Cat No. C2206, 1:2000). .. Brightfield scans of stained tissue were captured at 40x magnification using Nikon 3 microscope and associated ND acquisition software, with the help of the advanced imaging facility (AIF) at the University of Leicester.

    Article Title: Phosphorylations of serines 21/9 in glycogen synthase kinase 3α/β are dispensable for V600E BRAF -driven premalignant tumour development in the mouse intestine
    Article Snippet: .. Primary antibodies used for immunostaining were: BrdU (Cell signalling, 5292, 1:500), phospho-histone H3 (Cell signalling, 9701, 1:500) and β-catenin (Sigma, Cat No. C2206, 1:2000). .. Brightfield scans of stained tissue were captured at 40x magnification using Nikon 3 microscope and associated ND acquisition software, with the help of the advanced imaging facility (AIF) at the University of Leicester.

    Incubation:

    Article Title: REV1 Loss Triggers a G2/M Cell-Cycle Arrest Through Dysregulation of Mitotic Regulators
    Article Snippet: Primary and secondary antibodies were diluted in 5% BSA dissolved in 1× TBS with 0.01% Tween-20 (Fisher Scientific, Thermo Fisher Scientific, Waltham, MA, USA). .. The membranes were incubated overnight at 4 ◦C with the following primary antibodies: β-Actin (Cell Signaling Technologies, 13E5, Danvers, MA, USA) at 1:1000, Cyclin B1 (Cell Signaling Technologies, 4138T, Danvers, MA, USA) at 1:250, Phospho-Histone H3 (Cell Signaling Technologies, 9713, Danvers, MA, USA) at 1:250, Beta-Tubulin (Cell Signaling Technologies, 2128, Danvers, MA, USA) at 1:500, H3 (Cell Signaling Technologies, 4499, Danvers, MA, USA) at 1:250, and REV1 (Invitrogen, 48793, Carlsbad, CA, USA) at 1:1000. .. The membranes were washed four times in 1× TBST before incubation with a secondary antibody (LiCORBio 800CW Goat Anti-Rabbit, LI-COR Biosciences, Lincoln, NE, USA) at 1:20,000.

    Article Title: REV1 Loss Triggers a G2/M Cell-Cycle Arrest Through Dysregulation of Mitotic Regulators
    Article Snippet: Primary and secondary antibodies were diluted in 5% BSA dissolved in 1× TBS with 0.01% Tween-20 (Fisher Scientific, Thermo Fisher Scientific, Waltham, MA, USA). .. The membranes were incubated overnight at 4 °C with the following primary antibodies: ꞵ-Actin (Cell Signaling Technologies, 13E5, Danvers, MA, USA) at 1:1000, Cyclin B1 (Cell Signaling Technologies, 4138T, Danvers, MA, USA) at 1:250, Phospho-Histone H3 (Cell Signaling Technologies, 9713, Danvers, MA, USA) at 1:250, Beta-Tubulin (Cell Signaling Technologies, 2128, Danvers, MA, USA) at 1:500, H3 (Cell Signaling Technologies, 4499, Danvers, MA, USA) at 1:250, and REV1 (Invitrogen, 48793, Carlsbad, CA, USA) at 1:1000. .. The membranes were washed four times in 1× TBST before incubation with a secondary antibody (LiCOR-Bio 800CW Goat Anti-Rabbit, LI-COR Biosciences, Lincoln, NE, USA) at 1:20,000.

    Blocking Assay:

    Article Title: KRAS mutated lung adenocarcinoma responds to pan-ERBB and Aurora kinase inhibitors.
    Article Snippet: Lysates were loaded on 8–12% polyacrylamide gels (as appropriate), separated using an SDS-containing running buffer, and transferred to nitrocellulose membranes (Invitrogen) by semi-dry blotting. .. After blocking (5% BSA or milk in 1x TBS-T for 1 h at room temperature), membranes were probed with primary antibodies against phosphoEGFR (Y1068, Cell Signaling #2234), EGFR (Cell Signaling #4267), phospho-ERK1/2 (T202/Y204, Cell Signaling #4376), ERK1/2 (Cell Signaling #4695), phospho-Histone H3 (Ser10, Cell Signaling #9701), Histone H3 (Cell Signaling #4499), Cyclin B1 (Santa Cruz, sc-7393), phospho-Aurora A /Aurora B/ Aurora C (T288 /T232 /T198, Cell Signaling #2914), Aurora A (Santa Cruz sc-56881), Aurora B (Cell Signaling #3094), phospho-STAT3 (Y705, Cell Signaling #9131), STAT3 (Cell Signaling #9139), phospho-TBK1 (S172, Cell Signaling #5483), TBK1 (Cell Signaling #3504), phospho-p38 (T180/Y182, Cell Signaling #4511), p38 (Cell Signaling #8690), CDK6 (Santa Cruz sc56282), CDK4 (Santa Cruz sc-23896), Cyclin D1 (Santa Cruz sc8396), RB (Santa Cruz sc-102), phospho-RB (S807/811, Cell Signaling 9308), phospho-AKT (S473, Cell Signaling #9271) and AKT (Cell Signaling #9272). .. HSC70 (Santa Cruz sc-7298) and ß-Actin (Santa Cruz sc-47778) were used as a loading control.

    Article Title: KRAS mutated lung adenocarcinoma responds to pan-ERBB and Aurora kinase inhibitors
    Article Snippet: Lysates were loaded on 8–12% polyacrylamide gels (as appropriate), separated using an SDS-containing running buffer, and transferred to nitrocellulose membranes (Invitrogen) by semi-dry blotting. .. After blocking (5% BSA or milk in 1x TBS-T for 1 h at room temperature), membranes were probed with primary antibodies against phospho-EGFR (Y1068, Cell Signaling #2234), EGFR (Cell Signaling #4267), phospho-ERK1/2 (T202/Y204, Cell Signaling #4376), ERK1/2 (Cell Signaling #4695), phospho-Histone H3 (Ser10, Cell Signaling #9701), Histone H3 (Cell Signaling #4499), Cyclin B1 (Santa Cruz, sc-7393), phospho-Aurora A /Aurora B/ Aurora C (T288 /T232 /T198, Cell Signaling #2914), Aurora A (Santa Cruz sc-56881), Aurora B (Cell Signaling #3094), phospho-STAT3 (Y705, Cell Signaling #9131), STAT3 (Cell Signaling #9139), phospho-TBK1 (S172, Cell Signaling #5483), TBK1 (Cell Signaling #3504), phospho-p38 (T180/Y182, Cell Signaling #4511), p38 (Cell Signaling #8690), CDK6 (Santa Cruz sc-56282), CDK4 (Santa Cruz sc-23896), Cyclin D1 (Santa Cruz sc-8396), RB (Santa Cruz sc-102), phospho-RB (S807/811, Cell Signaling 9308), phospho-AKT (S473, Cell Signaling #9271) and AKT (Cell Signaling #9272). .. HSC70 (Santa Cruz sc-7298) and ß-Actin (Santa Cruz sc-47778) were used as a loading control.



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